- Open Access
Correction to: Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR
BMC Hematology volume 19, Article number: 13 (2019)
- The original article was published in BMC Hematology 2014 14:4
Correction to: BMC Hematol (2014) 14:4
The copy number of the HBA1 assay for the -(α)20.5 deletion in the HBA-CNV method described in the original article  was incorrectly reported. The authors wish to note that the HBA1 assay will not be affected by the –(α)20.5 deletion and will show two copies (Table 1 - corrected). The 3′ breakpoint of the –(α)20.5 deletion is located within exon 2 of the HBA1 gene , leaving intact the area where the HBA1 assay is amplifying. The partial deletion of HBA1 causes a complete abolition of the gene expression, hence –(α)20.5 is considered as a double gene deletion. This shows that even though the HBA1 assay may show two copies, a deletion affecting both alpha-globin genes can not be excluded. Similarly, the Hb Var database contains examples of deletions that will not influence HBA2 assay copy number despite affecting both alpha-globin genes. Hence, molecular data should always be evaluated together with hematological data.
Runa M, Grimholt RM, Urdal P, Piehler AP. Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR. BMC Hematol. 2014;14:4.
Nicholls RD, Higgs DR, Clegg JB, Weatherall DJ. Alpha zero-thalassemia due to recombination between the alpha 1-globin gene and an AluI repeat. Blood. 1985;65(6):1434–8.
About this article
Cite this article
Grimholt, R.M., Urdal, P., Klingenberg, O. et al. Correction to: Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR. BMC Hematol 19, 13 (2019). https://doi.org/10.1186/s12878-019-0144-5