- Research article
- Open Access
Systematic review of azacitidine regimens in myelodysplastic syndrome and acute myeloid leukemia
BMC Hematology volume 18, Article number: 3 (2018)
5-Azacitidine administered as a 7-day dosing regimen (7–0-0) is approved in high risk IPSS myelodysplastic syndrome (MDS) patients. Alternative regimens such as a 5-day (5–0-0) or 7-day with a weekend break (5–2-2) are commonly used. No randomized controlled trial has been done directly comparing all three dosing regimens. The objective of this study was to compare the efficacies of the 5–0-0, 5–2-2, and 7–0-0 regimens in MDS and AML.
A systematic review was conducted using MEDLINE, EMBASE and CENTRAL. Eligible studies were randomized controlled trials (RCTs), observational prospective and retrospective studies. The primary clinical outcomes were Objective Response Rate (ORR) defined as the sum of complete response (CR), partial response (PR), and hematological improvement (HI) as defined by the IWG 2006 criteria. A meta-analysis of simple proportions was conducted using a random effects model with weights defined according to Laird and Mosteller. Comparisons between groups were not attempted due to the heterogeneity of study designs.
The only RCT directly comparing alternative azacitidine regimens showed no difference in ORR between the 5–0-0 and 5–2-2 regimens. All other RCTs compared a dosing regimen to conventional care. The pooled proportion of ORR was 44.8% with 95% CI (42.8%, 45.5%) for 7–0-0, 41.2% with 95% CI (39.2%, 41.9%) for 5–0-0, and 45.8% with 95% CI (42.6%, 46.4%) for 5–2-2.
Indirect comparison of alternative azacitidine dosing regimens in MDS and AML shows a benefit for the 7-day regimen in attaining ORR. Additional RCTs are required to definitively address this comparison.
Azacitidine has become the standard of care for patients with high risk myelodysplastic syndrome (MDS) when a hematopoietic stem cell transplant is not an option. In the CALGB 9221 randomized clinical trial, azacitidine administered at 75 mg/m2 for 7 continuous days resulted in an objective response rate of 16% compared to no response in the control group [1, 2]. This response rate included improvement in peripheral cytopenias resulting in transfusion independence as well as a reduction in the bone marrow blast percentage . The subsequent international phase III open label randomized controlled trial (RCT) AZA-001 comparing azacitidine to conventional care that included low dose cytarabine, best supportive care or intensive chemotherapy showed a statistically significant survival benefit as well as a doubling in the time to progression to AML with azacitidine. The results of the AZA-001 clinical trial led to the FDA extending a survival benefit to the use of the drug in intermediate-2/high risk MDS by international prognostic scoring criteria (IPSS), CMML with 10–30% blasts, and AML with 20–30% blasts .
The standard approved dose cycle of azacitidine has been 75 mg/m2 for 7 continuous days (7–0-0), according to the AZA-001 and CALGB clinical trials [2, 3]. However, due to difficulties with administration of weekend doses, many centres either administer the same dose on a 5-day schedule (5–0-0), or a 5-day schedule followed by a weekend break followed by an additional 2 days (5–2-2) . There has been no formal randomized clinical trial comparing the efficacy and tolerability of the alternative azacitidine doses, and the assumption has been that they are equivalent . However, there are several important pharmacologic points that may challenge this assumption.
The active form of azacitidine binds both RNA and DNA, exerting its cytotoxic effect via interference with RNA transcription and DNA methyltransferase I activity in actively proliferating cells [1, 6]. In studies of azacitidine pharmacokinetics, the drug was undetectable in daily pretreatment blood samples, suggesting a rapid elimination and no accumulation . Therefore, as the drug is only active in proliferating cells and does not accumulate, shorter durations of therapy within each cycle are less likely to have the drug encounter all malignant clones in their S-phase [1, 5]. This would conceptually argue for the increased efficacy of longer duration of treatment per cycle . However, this argument does not discount interrupted courses of therapy such as 5–2-2. Since the benefit of azacitidine has most definitively been demonstrated in RCT using the 7–0-0 schedule, it becomes important to collect efficacy data on the alternative dosing schedules in order to ensure they are at least as equally effective as 7–0-0 . The objective of the current systematic review is to evaluate the efficacy and tolerability of the 5–0-0, 5–2-2, and 7–0-0 azacitidine dosing regimens in MDS patients.
The primary outcome was objective response rate (ORR) calculated as the combination of complete response (CR), partial response (PR), and hematological improvement (HI) as per the IWG 2006 criteria . Due to the heterogeneity of the reporting of outcome data, the ORR was determined to be the outcome that could be extracted from the greatest number of articles and abstracts describing azacitidine treatment. Due to the inability to separate the treatment outcomes of AML patients that were included in retrieved studies of azacitidine therapy in MDS, these patients were included in the analysis of ORR.
A systematic literature search was conducted in November 2014 and updated in October 2015 using the OVID interface and included MEDLINE, EMBASE, and Cochrane Central Register of Controlled Trials (Central) databases. The full methodology is described below with no additional review protocol or registration. No language restrictions were applied.
A sensitive search strategy was based on combination of subject headings and text-words using alternative spellings and word endings, such as but not limited to the search terms ‘AZA’, ‘azacitidine’, ‘azacytidine’, ‘vidaza’, ‘ladakamycin’, ‘myelodysplastic syndrome’, ‘myelodysplasia’, and ‘MDS’. Modifications to the search strategy were made for each database using appropriate thesaurus terms and fields. The Medline search strategy is indicated in Additional file 1. Articles were evaluated for inclusion based on the title and abstract. If an abstract was not available, an attempt was made to retrieve the full article for evaluation. Any articles retrieved with the search that included AML and CMML patients were included in the subsequent analysis if they met inclusion criteria.
Assessment of study quality and data extraction
Articles meeting inclusion criteria were retrieved for full data extraction. The inclusion criteria were as follows: randomized clinical trials, observational prospective, and observational retrospective studies evaluating the clinical response of patients with myelodysplastic syndrome to azacitidine. Studies were excluded if they were phase I clinical trials, review articles, case series, or abstracts that were subsequently published in full form. Studies assessing AML and CMML patients retrieved with the search strategy were included in the analysis. Relevant data from included articles was extracted using a data collection form, and encompassed the disease characteristics of patients included in selected studies, vidaza dosing regimens used, and outcome variables. The primary outcome was objective response rate (ORR) calculated as the combination of complete response (CR), partial response (PR), and hematological improvement (HI) as per the International Working Group (IWG) criteria. In those publications where the ORR was directly reported as is defined by the IWG criteria, this ORR was recorded. In those publications which did not directly report the ORR but did report CR, PR, and/or HI as defined by the IWG, the ORR was calculated as the sum of available data. If the ORR could not be calculated from an abstract and/or article, then this publication was not included in the data analysis. Articles that reported objective response based on the IWG 2000 criteria were included in the analysis, with the justification based on the overall similarity in objective response using the two criteria as is shown in Additional file 2: Table S3. Articles that included only AML patients had objective response defined as CR + CRi + PR + HI, where HI referred to patients who did not attain the response criteria for CR/CRi or PR. Articles that reported on MDS and AML patients where the objective response could not be separated based on disease type were included in the analysis. If the outcome results reported in a publication could not be attributed to a particular dosing regimen of azacitidine, an attempt was made to contact the corresponding author in order to obtain this data. The quality of RCT, including any possible degree of bias in the study, was assessed according to the criteria proposed by Jadad et al.  Non-randomized observational studies were assessed with respect to attrition bias and reporting bias using the Cochrane Bias Assessment Tool .
A meta-analysis of effect sizes of the articles meeting inclusion criteria was planned but could not be performed as there were insufficient RCT directly comparing the efficacy of various azacitidine dosing regimens. A pooled proportion analysis using a random effects model was conducted as previously described [11, 12]. The primary outcomes of interest were objective response rate and complete response as per IWG . A z-test was used to assess for differences between effects, with a p-value <0.05 considered statistically significant. A sensitivity analysis was done evaluating the pooled proportion of ORR in subgroups of patients retrieved with the search strategy.
Results of the search strategy from the systematic review
The search strategy from all databases identified 1690 articles and abstracts after duplicates were removed, from which 47 articles and 90 abstracts met inclusion criteria for full study evaluation (Fig. 1). Of the 47 articles, there were 6 that did not report outcomes corresponding to individual azacitidine dosing regimens thereby excluding these articles from the final analysis. One article was excluded because the dosing regimen did not correspond to any of 5–0-0, 5–2-2, or 7–0-0, and another two articles were excluded because ORR could not be calculated. The remaining 38 articles along with the 90 abstracts were included in the pooled proportions analysis. As there were no randomized controlled trials directly comparing alternative dosing schedules of azacitidine, a meta-analysis of effects could not be performed. For most domains, studies had an unclear or a high risk of bias (Fig. 2). References to studies not cited in the article text but included in the data analysis are shown in Additional file 3.
Characteristics of included studies
Of the 128 articles and abstracts meeting inclusion criteria, there were a total of 3 articles detailing randomized controlled trials (RCT) with one of the three articles summarizing data from three previous CALGB clinical trials (Table 1) . Two of the RCTs evaluated the 7–0-0 regimen and one of the RCTs evaluated the 5–0-0 and 5–2-2 regimens, with all RCTs comparing azacitidine to conventional care that includes one or more of best supportive care, therapy with Ara-C, or intensive chemotherapy. The remainder of the articles were observational studies with either prospective (11/38) or retrospective (24/38) design.
A summary of the patient characteristics of included studies is shown in Additional file 2: Table S1. There were a total of 7520 patients, with 5545 patients receiving the 7–0-0 regimen, 1207 receiving the 5–0-0 regimen, and 768 receiving the 5–2-2 regimen. The median age of all patients was 70. The median age of all patients reported in articles with the 5–0-0, 5–2-2, and 7–0-0 regimens was 66, 72, and 69, respectively. The mean number of cycles received by patients treated with the 5–0-0, 5–2-2, and 7–0-0 regimens was 6, 6.7, and 5.5, respectively. An r by c chi square test was done showing that it was statistically more likely for patients receiving the 7–0-0 treatment regimen to have IPSS high risk than it was for the other treatment groups χ2(2, N = 2050) = 13.33, p = 0.0013. Similarly, it was statistically more likely for patients receiving the 7–0-0 treatment regimen to have a diagnosis of AML χ2(2, N = 2760) = 121.4, p < 0.000001. For articles that reported ECOG values, those articles reporting on patients treated with the 5–0-0, 5–2-2, and 7–0-0 regimens had 80%, 75%, and 80% of their patients in an ECOG <= 1 group, respectively. An r by c chi square test was done showing that there was no statistically significant association between the proportion of patients with ECOG score ≤ 1 and treatment regimen χ2(2, N = 1825) = 0.681, p = 0.7114.
Direct comparison of dosing regimens
The outcomes from the articles included in the systematic review are summarized in Additional file 2: Table S2. There was a small number of studies directly comparing the different azacitidine regimens. There were two studies directly comparing the 5–0-0 and 5–2-2 dosing regimens. One was a randomized controlled trial showing no statistically significant difference in ORR between the 5–0-0 and 5–2-2 dosing regimens . The other was an observational retrospective study that also showed no difference in ORR between the two regimens . There were also two studies directly comparing the 5–0-0 and 7–0-0 regimens, both of which are observational retrospective studies that showed no statistically significant difference in ORR between the two regimens [15, 16]. Due to methodological heterogeneity, we did not conduct a direct comparison between groups.
Results of the pooled proportions analysis
The pooled proportion of ORR was 44.8% (95% CI 42.8% to 45.5%) for the 7–0-0 dosing regimen, 41.2% (95% CI 39.2% to 41.9%) for the 5–0-0 regimen, and 45.8% (95% CI 42.6% to 46.4%) for the 5–2-2 regimen. A sensitivity analysis was done evaluating the pooled proportion of ORR in subgroups of patients such as those strictly reported to have a diagnosis of MDS, to have higher risk disease based on the IPSS, and based on the type of study that was performed (observational prospective, observational retrospective, RCT). Results of the sensitivity analysis are shown in Table 2.
This systematic review was intended to test the hypothesis of whether the more practically convenient 5–0-0 and 5–2-2 azacitidine dosing regimens used to treat MDS have at least equivalent efficacy to the approved 7–0-0 dosing regimen studied in randomized clinical trials. The number of studies directly comparing the alternative dosing regimens was small, and no study directly compared all three regimens to each other. In those studies where a comparison of alternative regimens was made, they were found to be equivalent in terms of the ORR. Unfortunately, methodological heterogeneity of studies prevented a meta-analysis of effects.
The choice of ORR as the primary outcome variable was made due to the heterogeneity of the reporting of outcome data in articles describing azacitidine therapy. The ORR was determined to be able to pool data from the greatest amount of published literature on the subject of azacitidine dosing. Due to the heterogeneity of the reporting of survival data and to the substantial number of articles and abstracts that did not report overall survival, this outcome could not be used in the pooled proportions analysis. Other outcome variables commonly reported in studies of azacitidine, including CR, PR, HI, and transfusion dependence were independently evaluated as potential primary outcome variables for the pooled proportions analysis, and none encompassed as many studies as the ORR. Only the reporting of CR was similar to that of the ORR, with far more heterogeneity of reporting noted for the other outcome variables. Similarly to ORR, the attainment of stable disease has been found to have a correlation with overall survival in MDS, and was considered for inclusion in the pooled proportion analysis . However, stable disease as an outcome was reported in a total of 42% of articles and abstracts. The exclusion of all articles and abstracts not reporting on stable disease would result in a greater degree of bias affecting the interpretation of the outcomes.
The definition of ORR did undergo an update in 2006, resulting in differences in the way this value was calculated from patient data compared to preceding years [8, 18]. Although this is a limitation of choosing the ORR as an outcome variable, the majority (75%) of the ORR used in the pooled proportions analysis was determined using the IWG 2006 criteria. Furthermore, due to the significant similarities between the IWG 2000 and IWG 2006 definitions of ORR, the relatively small number of articles and abstracts included in this review that reported the ORR using the IWG 2000 definition is unlikely to significantly affect the pooled proportions analysis. Additional file 2: Table S3 compares the IWG 2000 and IWG 2006 criteria for ORR.
The inclusion of CMML and AML patients in this systematic review was required because it was not possible to separate the outcomes of these patients from the MDS patients in most studies. Excluding any study that reported on CMML or AML in addition to MDS would have resulted in a substantial reduction in the total number of studies and patients as is shown in the sensitivity analysis (Table 2). For studies that reported on AML patients included in the review, response outcomes were reported allowing for the determination of ORR [19, 20,21,22]. Two studies of AML patients retrieved with the search strategy that did not report on HI were excluded from the pooled proportion and sensitivity analyses because ORR could not be calculated.
A pooled proportions analysis of the different dosing regimens across both randomized and observational studies was performed. Understanding the inherent limitation of this analysis [11, 12], it was found that the 7–0-0, 5–2-2, and 5–0-0 regimens had pooled ORR of 44.8%, 45.8%, and 41.2%, respectively. Interestingly, the confidence intervals of the 7–0-0 and 5–0-0 regimens do not overlap in a random effects model of pooled proportions, suggesting the possibility that the 7–0-0 may have somewhat greater efficacy in terms of the ORR than the 5–0-0 regimen. This as an indirect comparison of pooled ORR, but lends support to the idea that total time of exposure to azacitidine does play a role in clinical efficacy . The same outcome is noted for the indirect comparison of the ORR of the 5–2-2 regimen and 5–0-0 regimen, also suggesting the possibility that a longer exposure to azacitidine has clinical benefit. Indirect comparison of the 7–0-0 and 5–2-2 regimens yielded overlapping confidence intervals, suggestive of the equal efficacy of these regimens in terms of ORR. What seems to be consistent is that a total course of 7 days (with or without a weekend break) of treatment with azacitidine has a statistically significant higher pooled ORR than a 5-day course.
It is important to note that the pooled set of patients receiving the 7–0-0 treatment regimen had a greater proportion of patients with IPSS high risk score and a diagnosis of AML than the other two treatment regimens. This likely reflected the fact that the 7–0-0 regimen was studied in clinical trials and is the regimen receiving clinical approval. How this impacted the pooled ORR for this group of patients across all studies is not clear because the IPSS is a prognostic score predictive of survival in MDS, not objective response rate . To determine whether the higher proportion of AML patients treated with the 7–0-0 regimen affected the outcome of the pooled proportion analysis, a sensitivity analysis was performed assessing the response of patients with a diagnosis of MDS only (studies assessing any patients with a diagnosis of AML or CMML were excluded). It yielded the same outcome in that the pooled ORR with the 7–0-0 and 5–2-2 regimens were higher than the pooled ORR with the 5–0-0 regimen. The slightly higher ORR of the 7–0-0 in relation to the 5–0-0 regimen in an indirect pooled proportional analysis was consistent in similar sensitivity analyses focusing on patients assessed in randomized clinical trials, and on patients assessed in prospective observational studies (Table 2). The clinical significance of this finding is uncertain, however, without a direct comparison of the different dosing regimens in a clinical trial.
An important limitation of the current systematic review is that due to a paucity of randomized controlled trials in directly comparing the alternative azacitidine dosing regimens, most of the articles and abstracts included in this systematic review refer to observational prospective and retrospective studies. With a lack of randomization, blinding, and allocation concealment in these studies, there is a substantial risk of selection, performance, and detection bias as summarized in Fig. 2 . However, with the consistency of the finding that the pooled ORR for a total of 7 days of azacitidine exposure is higher that the pooled ORR for 5 days of exposure, a randomized clinical trial is required for direct comparison and a definitive answer. If a trial is not performed, a standardization of outcome data reporting in the literature would facilitate the update of the sort of analysis done in this study with the inclusion of stable disease and survival as outcomes.
In summary, this systematic review of alternative azacitidine dosing regimens in MDS and AML patients has highlighted an important deficiency in the literature regarding outcome reporting. Based on a small number of studies directly comparing alternative dosing regimens, there is no difference in efficacy of the 7–0-0, 5–2-2, and 5–0-0 dosing regimens in attaining ORR. However, an indirect comparison of the dosing regimens in the form of a pooled proportions analysis encompassing all studies on the subject yielded a slightly higher ORR for a total of 7 days of exposure to azacitidine as compared to 5 days. A prospective randomized clinical trial directly comparing the three dosing regimens is required to definitively address this comparison. Furthermore, a standardization of the reporting of outcomes of azacitidine treatment would facilitate future indirect comparisons of dosing regimens if a randomized trial is not preformed.
AMS M, Florek M. 5-Azacytidine. In: Small molecules in oncology. 2nd ed. New York: Springer Heidelberg; 2014. p. 299–324.
Silverman LR, Demakos EP, Peterson BL, Kornblith AB, Holland JC, Odchimar-Reissig R, Stone RM, Nelson D, Powell BL, DeCastro CM, Ellerton J, Larson RA, Schiffer CA, Holland JF. Randomized controlled trial of Azacitidine in patients with the Myelodysplastic syndrome: a study of the cancer and leukemia group B. J Clin Oncol. 2002;20:2429–40.
Fenaux P, Mufti GJ, Hellstrom-Lindberg E, Santini V, Finelli C, Giagounidis A, Schoch R, Gattermann N, Sanz G, List A, Gore SD, Seymour JF, Bennett JM, Byrd J, Backstrom J, Zimmerman L, McKenzie D, Beach CL, Silverman LR, for the International Vidaza High-Risk MDS Survival Study Group. Efficacy of azacitidine compared with that of conventional careregimens in the treatment of higher-risk myelodysplasticsyndromes: a randomised, open-label, phase III study. Lancet Oncol. 2009;10:223–32.
Frame D. Alternative dosing schedules for methylation inhibitors in MDS treatment. Managed Care. 2009;18(11):S15–20.
Saunthararajah Y. Key clinical observations after 5-azacytidine and decitabine treatment of myelodysplastic syndromes suggest practical solutions for better outcomes. Hematology. 2013; https://doi.org/10.1182/asheducation-2013.1.511.
Keating GM. Azacitidine. Drugs. 2012;72(8):1111–36.
Santini V, Prebet T, Fenaux P, Gattermann N, Nilsson L, Pfeilstocker M, Vyas P, List AF. Minimizing risk of hypomethylating agent failure in patients with higher-risk MDS and practical management recommendations. Leuk Res. 2014;38:1381–91.
Cheson BD, Greenberg PL, Bennett JM, Lowenberg B, Wijermans PW, Nimer SD, Pinto A, Beran M, de Witte TM, Stone RM, Mittelman M, Sanz GF, Gore SD, Schiffer CA, Kantarjian H. Clinical application and proposal for modification of the international working group (IWG) response criteria in myelodysplasia. Blood. 2006;108(2):419–25.
Jadad AR, Moore RA, Carroll D, et al. Assessing the quality of reports of randomized clinical trials: is blinding necessary? Control Clin Trials. 1996;17:1–12.
JPT H, Altman DG, Sterne JAC. Chapter 8: assessing risk of bias in included studies. In: JPT H, Green S, editors. Cochrane handbook for systematic reviews of interventions. Version 5.1.0 [updated march 2011]. The Cochrane collaboration; 2011. http://handbook-5-1.cochrane.org/. Accessed 1 June 2016.
Stukel TA, Demidenko E, Dykes J, Karagas MR. Two-stage methods for the analysis of pooled data. Statist Med. 2001;20:2115–30.
Lazo-Langner A, Rodger MA, Barrowman NJ, Ramsay T, Wells PS, Coyle DA. Comparing multiple competing interventions in the absence of randomized trials using clinical risk-benefit analysis. BMC Med Res Methodol. 2012;12:3.
Silverman LR, McKenzie DR, Peterson BL, Holland JF, Backstrom JT, Beach CL, Larson RA. Further analysis of trials with Azacitidine in patients with Myelodysplastic syndrome: studies 8421, 8921, and 9221 by the cancer and leukemia group B. J Clin Oncol. 2006;24(24):3895–903.
Lyons RM, Cosgriff TM, Modi SS, Gersh RH, Hainsworth JD, Cohn AL, McIntyre HJ, Fernando IJ, Backstrom JT, Beach CL. Hematologic response to three alternative DosingSchedules of Azacitidine in patients with Myelodysplastic syndromes. J Clin Oncol. 2009;27(11):1850–6.
Xicoy B, Jiménez MJ, García O, et al. Results of treatment with azacitidine in patients aged 75 years included in the Spanish registry of Myelodysplastic syndromes. Leuk Lymphoma. 2014;55(6):1300–3.
García-Delgadoa R, de Miguelb D, Bailénc A, et al. Effectiveness and safety of different azacitidine dosage regimens inpatients with myelodysplastic syndromes or acute myeloid leukemia. Leuk Res. 2014;38:744–50.
Gore SD, Fenaux P, Santini V, et al. A multivariate analysis of the relationship between response and survival among patients with higher-risk myelodysplastic syndromes treated within azacitidine or conventional care regimens in the randomized AZA-001 trial. Haematologica. 2013;98:1062–72.
Cheson BD, Bennett JM, Kantarjian H, et al. Report of an international working group to standardize response criteria for myelodysplastic syndromes. Blood. 2000;96:3671–4.
O’Reilly MA, McHale C, Almazmi A, et al. A 5-day outpatient regimen of azacitidine is effective and well tolerated in patients with acute myeloid leukemia unsuitable for intensive chemotherapy. Leuk Lymphoma. 2014;55(12):2950–1.
Passweg JR, Pabst T, Blum S, et al. Azacytidine for acute myeloid leukemia in elderly or frail patients: a phase II trial (SAKK 30/07). Leuk Lymphoma. 2014;55(1):87–91.
van der Helm LH, Veeger NJGM, M. Kooy VM, et al. Azacitidine results in comparable outcome in newly diagnosed AML patients with more or less than 30% bone marrow blasts. Leuk Res. 2013;37:877–82.
van der Helm LH, Scheepers ERM, Veeger NJGM, et al. Azacitidine might be beneficial in a subgroup of older AML patients compared to intensive chemotherapy: a single centre retrospective study of 227 consecutive patients. J Hematol Oncol. 2013;6:29–37.
Greenberg P, Cox C, LeBeau MM, et al. International scoring system for evaluating prognosis in myelodysplastic syndromes. Blood. 1997;89(6):2079–88.
Sadashiv SK, Hilton C, Khan C, et al. Efficacy and tolerability of treatment with azacitidine for 5 days in elderly patients with acute myeloid leukemia. Cancer Medicine. 2014;3(6):1570–8.
Minoia C, Sgherza N, Loseto G, et al. Azacitidine in the front-line treatment of therapy-related myeloid Neoplasms: a multicenter case series. Anticancer Res. 2015;35:461–6.
Drummond MW, Pocock C, Boissinot M, et al. A multi-centre phase 2 study of azacitidine in chronic myelomonocytic leukaemia. Leukemia. 2014;28:1570–2.
Fianchi L, Criscuolo M, Breccia M, et al. High rate of remissions in chronic myelomonocytic leukemia treated with 5-azacytidine: results of an Italian retrospective study. Leuk Lymphoma. 2013;54(3):658–61.
Ballya C, Adèsa L, Rennevilleb A, Seberta M, et al. Prognostic value of TP53 gene mutations in myelodysplasticsyndromes and acute myeloid leukemia treated with azacitidine. Leuk Res. 2014;38:751–5.
Breccia M, Loglisci G, Salaroli A, et al. 5-Azacitidine efficacy and safety in patients aged > 65 years with myelodysplastic syndromes outside clinical trials. Leuk Lymphoma. 2012;53(8):1558–60.
Breccia M, Loglisci G, Cannella L, et al. Application of French prognostic score to patients with InternationalPrognostic scoring system intermediate-2 or high risk myelodysplasticsyndromes treated with 5-azacitidine is able to predict overall survivaland rate of response. Leuk Lymphoma. 2012;53(5):985–6.
Douvali E, Papoutselis M, Vassilakopoulos TP, et al. Safety and efficacy of 5-azacytidine treatment in myelodysplastic syndrome patients with moderate and mild renal impairment. Leuk Res. 2013;37:889–93.
Duong VH, Lancet JE, Alrawi E, et al. Outcome of azacitidine treatment in patients with therapy-related myeloid neoplasms with assessment of prognostic risk stratification models. Leuk Res. 2013;37:510–5.
Ettou S, Audureau E, Humbrecht C, et al. Fas expression at diagnosis as a biomarker of azacitidine activityin high-risk MDS and secondary AML. Leukemia. 2012;26:2297–9.
Fianchi L, Criscuolo M, Lunghi M, et al. Outcome of therapy-related myeloid neoplasms treated with azacitidine. J Hematol Oncol. 2012;5:44.
Fili C, Malagola M, Follo MY, et al. Prospective phase II study on 5-days Azacitidine forTreatment of symptomatic and/or erythropoietin unresponsive patients with low/INT-1–risk Myelodysplastic syndromes. Clin Cancer Res. 2013;19(12):3297–308.
Gryna J, Zeigler ZR, Shadduck RK, et al. Treatment of myelodysplastic syndromes with 5-azacytidine. Leuk Res. 2002;26:893–7.
Itzykson R, Kosmider O, Cluzeau T, et al. Impact of TET2 mutations on response rate to azacitidine in myelodysplastic syndromesand low blast count acute myeloid leukemias. Leukemia. 2011;25:1147–52.
Itzykson R, Thépot S, Quesnel B, et al. Prognostic factors for response and overall survival in 282 patients with higher-risk myelodysplastic syndromes treated with azacitidine. Blood. 2011;117:403–11.
Lee JH, Choi Y, Kim SD, et al. Comparison of 7-day azacitidine and 5-day decitabine for treating myelodysplastic syndrome. Ann Hematol. 2013;92:889–97.
Lee YG, Kim I, Yoon SS, et al. Comparative analysis between azacitidine and decitabine forthe treatment of myelodysplastic syndromes. Br J Hematol. 2013;161:339–47.
Al-Ali HK, Jaekel N, Junghanss C, et al. Azacitidine in patients with acute myeloid leukemia medically unfit for or resistant to chemotherapy: a multicenter phase I/II study. Leuk Lymphoma. 2012;53(1):110–7.
Martin MG, Walgren RA, Procknow E, et al. A phase II study of 5-day intravenous azacitidine in patients with myelodysplastic syndromes. Am J Hematol. 2009;84:560–4.
Moon JH, Kim SN, Kang BW, et al. Predictive value of pretreatment risk group and baseline LDH levels in MDS patients receiving azacitidine treatment. Ann Hematol. 2010;89:681–9.
Müller-Thomas C, Schuster T, Peschel C, Götze KS. A limited number of 5-azacitidine cycles can be effective treatment in MDS. Ann Hematol. 2009;88:213–9.
Müller-Thomas C, Rudelius M, Rondak IC, et al. Response to azacitidine is independent of p53 expression in higher-risk myelodysplastic syndromesand secondary acute myeloid leukemia. Haematologica. 2014;99:e179.
O’Reilly MA, McHale C, Almazmi A, et al. A 5-day: the favourable way? Ann Hematol. 2014;93:1619–20.
Ozbalak M, Cetiner M, Bekoz H, et al. Azacitidine has limited activity in ‘real life’ patientswith MDS and AML: a single centre experience. Hematol Oncol. 2012;30:76–81.
Papoutselis M, Douvali E, Papadopoulos V, et al. Has introduction of azacytidine in everyday clinical practice improved survival in late-stage Myelodysplastic syndrome? A single center experience. Leuk Res. 2014;38:161–5.
Pierdomenico F, Esteves S, Almeida A. Efficacy and tolerability of 5-day azacytidine dose-intensified regimen in higher-risk MDS. Ann Hematol. 2013;92:1201–6.
Tobiasson M, Dybedahl I, Holm MS, et al. Limited clinical efficacy of azacitidine in transfusion-dependent, growth factor-resistant, low- and Int-1-risk MDS: results from the nordic NMDSG08A phase II trial. Blood Cancer J. 2014;4:1–7.
Diamantopoulos P, Zervakis K, Papadopoulou V, et al. 5-Azacytidine in the treatment of Intermediate-2 and high-risk Myelodysplastic syndromes and acute myeloid leukemia. A five-year experience with 44 consecutive patients. Anticancer Res. 2015;35:5141–8.
Authors are kindly indebted to Alla Iansavitchene, BSc, MLIS, Library Services, London Health Science Centre, Western University, Ontario, Canada, for her help with the development and conduct of the literature search.
We thank Dr. Antonio Medina Almeida for providing the raw data from his retrospective study. We thank Dr. Maria Teresa Voso for providing us with her manuscript on azacitidine dosing.
Availability of data and materials
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Ethics approval and consent to participate
Not applicable to this study.
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Dr. Alejandro Lazo-Langner is the Hemostasis, Thrombosis and Vascular Biology Section Editor for BMC Hematology.
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This file includes the Medline search strategy used. (DOCX 13 kb)
This file includes Tables S1, S2, and S3. The former describes the available characteristics of all patients included in this study that were reported in literature articles (abstracts did not provide sufficient information regarding baseline patient characteristics). Table S2. describes available objective response rate and survival data from literature articles retrieved for this systematic review. Table S3. is a comparison of the IWG 2000 and IWG 2006 objective response criteria. (DOCX 31 kb)
This file includes the supplementary reference list for the systematic review. These references include articles and abstracts retrieved for this systematic review that were not cited in the main manuscript, but that are included in the data analysis. (DOCX 20 kb)
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Shapiro, R.M., Lazo-Langner, A. Systematic review of azacitidine regimens in myelodysplastic syndrome and acute myeloid leukemia. BMC Hematol 18, 3 (2018). https://doi.org/10.1186/s12878-017-0094-8