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Fig. 3 | BMC Hematology

Fig. 3

From: A quantitative assessment of the content of hematopoietic stem cells in mouse and human endosteal-bone marrow: a simple and rapid method for the isolation of mouse central bone marrow

Fig. 3

Comparisons of mouse cBM isolations using the single-cut and double-cut methods. The time required to isolate cBM by the two methods is shown for both the femur and tibia (a) and for each of the long bones individually (b). The number of cells isolated by the two methods from both long-bones is shown in (c) and cell yields for the individual bones are shown in (d). The percentages of tibial and femoral BM cells recovered from BALB/cJ (n = 19) and C57BL/6J (n = 37) mice are shown (e). The median number of cells recovered from each bone using the single-cut method are indicated in the legends. Cell yields are shown by investigator in (f). Refer to Table 1 for the number of harvests performed and the level of experience of the investigator

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