Skip to main content
Download PDF

Patan hospital experience in treating philadelphia chromosome/BCR-ABL1 positive chronic myeloid leukemia patients with gleevec (imatinib mesylate); the first generation specific tyrosine kinase inhibitor

BMC Hematology volume 10, Article number: 8 (2010) Cite this article

Abstract

Background

Chronic Myeloid Leukemia (CML) is caused by the abnormal fusion protein BCR-ABL1, a constitutively active tyrosine kinase and product of the Philadelphia chromosome. Gleevec (Imatinib mesylate) is a selective inhibitor of this kinase. Treatment with this agent is known to result in hematologic, cytogenetic, and molecular responses. Patan hospital (Patan, Nepal) is one of the Gleevec International Patient Assistance Program (GIPAP) centers for patients with CML.

Methods

A total of 106 Philadelphia positive CML patients were enrolled in our center between Feb 2003 and Jun 2008, and 103 of them were eligible for cytogenetic and/or hematologic response analyses.

Results

Out of 103 patients, 27% patients underwent cytogenetic analysis. Imatinib induced major cytogenetic responses in 89% and complete hematologic responses in almost 100% of the patients with confirmed CML. After a mean follow up of 27 months, an estimated 90% of the patients on imatinib remained in hematologic remission and more than 90% of the patients are still alive. About 30% of patients developed some form of manageable myelosuppression. A few patients developed non-hematologic toxic side effects such as edema and hepatotoxicity.

Conclusions

Our study demonstrates that imatinib is safe to use in a developing country. Furthermore, we demonstrate that imatinib is very effective and induced long lasting responses in a high proportion of patients with Ph chromosome/BCR-ABL1 positive CML. Imatinib is well tolerated by our patients. The lack of cytogenetic analysis in the majority of our patients hindered our ability to detect inadequate responses to imatinib and adjust therapy appropriately.

Peer Review reports

Background

Chronic myeloid leukemia (CML), is a myeloproliferative disorder caused by a translocation between chromosomes 9 and 22 that results in the Philadelphia (Ph) chromosome [t(9;22) (q34;q11)] [1]. The Ph chromosome was found to encode a reconstituted fusion gene known as BCR-ABL1, which is the principal cause of CML[2]. The BCR-ABL1 fusion gene encodes the BCR-ABL1 oncoprotein. This oncoprotein, which is constitutively active due to its uncontrolled tyrosine kinase activity, activates signal transduction pathways that lead to DNA replication and cell proliferation[3–5].

The incidence of CML is similar in countries around the world and may affect ethnic groups equally. The annual incidence of CML in the US is 1.6 cases per 100,000 adults (approximately 5000 new cases per year), with a male-to-female ratio of 1.4 to 1[6, 7]. The median age at diagnosis is approximately 55 years, with fewer than 10% of patients under the age of 20 years[8]. During the chronic phase of the disease there is clonal expansion of myeloid cells which continue to differentiate. Over time, however, the leukemic clone loses their ability to differentiate, and the disease ultimately progresses to blast crisis[3, 9, 10].

Until the 1980s, CML was regarded as incurable and thus inevitably fatal. Only some selected patients could afford treatment with, and in many cases were cured by allogeneic stem cell transplantation. However, because of the scarcity of matching donors and the high incidence of potentially fatal graft-versus-host disease in older patients, allogeneic stem cell transplantation is often not a feasible treatment strategy. Imatinib[11–16], is a potent first generation selective inhibitor of tyrosine kinase activity of BCR-ABL1. It was first approved by the Food and Drug Administration for CML in May 2001 and has been proven to be one of the most effective medications in achieving hematologic and cytogenetic remissions. Imatinib is a major break-through in the management of CML and has become the standard first line treatment strategy in the management of CML[17].

Patan hospital is the first Gleevec International Patient Assistance Program (GIPAP) center in Nepal. GIPAP was introduced by Dr. Zimmerman, the medical director of this hospital at that time, for patients with Ph chromosome/BCR-ABL1 positive CML since February 2003. Under this program patients with Philadelphia chromosome/BCR-ABL1 positive CML who cannot afford Imatinib receive it free of cost.

Methods

Study Design

Patients with Ph Chromosome/BCR-ABL1 positive CML at any stage of the disease were eligible for enrolment in GIPAP irrespective of prior therapy. Patients diagnosed with CML by blood and by bone marrow evaluation in our hospital, or referred from other hospitals, were sent for Ph chromosome/BCR-ABL1 analysis. All positive cases were enrolled in GIPAP. Patients who were diagnosed with CML and found to be Ph chromosome/BCR-ABL1 positive in other hospitals and referred to our hospital were also enrolled in GIPAP. Hydroxycarbamide and other medications for the treatment of CML were tapered off over a few days. Before commencement of the treatment with imatinib patients were screened for adequate renal and hepatic function, along with assessment of their performance status. Written informed consent was obtained from all patients before they were enrolled in GIPAP. Ethical approval was not considered necessary as GIPAP made available treatment that was already considered standard of care.

Patients were assigned to receive imatinib 400 mg/day for chronic phase and 600 mg/day for accelerated phase and blast crisis. Patients received continuous therapy unless unacceptable adverse effects or disease progression occurred. No other anticancer therapy was given. All patients received allopurinol until the total WBC count was 20,000/mL or less.

Complete blood counts and liver function tests were obtained once a week for the first month, every two weeks for next 2 months, and every 6 weeks afterwards. Patients were monitored for any adverse effects, clinical, and hematologic responses. Cytogenetic analyses were done for those patients who could afford this test, and patients who developed resistance to imatinib underwent mutation analysis. Only three patients underwent mutation analysis. Standard protocol for managing adverse effects during imatinib therapy was followed.

Assessment of Toxicity and Response

Safety assessments included the evaluation of adverse events and vital signs, hematological tests, biochemical tests, and physical examination. Toxicity was graded in accordance with the Common Toxicity Criteria of the National Cancer Institute.

Results

Patient Enrolment and Characteristics

Enrolment in GIPAP was started in Feb 2003. During the period from February 2003 to June 2008, a total of 106 patients ranging from age 10 to 71 years (median 39) were enrolled in GIPAP in our hospital. Starting with the first patient in February 2003, the enrolment steadily increased over the years. The most patients were enrolled in 2006 and 2007, with 26 and 23 patients in each of those years respectively. Most of the patients were from the Kathmandu and Lalitpur districts. There were patients from 37 of the 75 districts of Nepal. There were 70 males and 36 female patients (Table 1).

Table 1 Patient characteristics
Full size table

The majority of the patients were in the 31-50 years age group (51%) (Table 2). Of the total 106 patients, 29 patients were diagnosed at Patan Hospital and the rest (77 patients) were referred from other hospitals for GIPAP assistance. Of the 77 referred patients 36 patients were diagnosed by blood and/or bone marrow in different hospitals in Nepal and required additional investigations including Ph chromosome/BCR-ABL1 translocation status. The remaining 41 patients were referred from other hospitals for GIPAP enrolment after confirmation with Ph chromosome/BCR-ABL1 translocation analysis.

Table 2 GIPAP enrolment by age groups
Full size table

Of the total 106 patients, three patients were excluded from the study and the remaining 103 patients were eligible for analysis. The three patients excluded were: one who died before starting imatinib, one who registered but did not show up afterwards, and one who registered but then moved to another country. Among the 28 patients who were diagnosed at Patan hospital, only 10 patients presented with abdominal complaints such as abdominal fullness, swelling, mass, early satiety, abdominal discomfort or pain. The remaining patients presented with other diverse complaints.

Of the 103 patients, 96 patients were in chronic phase, 5 patients were in accelerated phase, and two patients were in blast crisis at the time of presentation. During enrolment, there were 61 patients on hydroxycarbamide, one on busulfan, and one on imatinib. Two other patients were taking combination therapy; one with hydroxycarbamide and imatinib and the other one was on hydroxycarbamide with busulfan (Table 3). The mean follow up was 27 months (median 24, range 1-66). Twenty-nine patients had repeated quantitative Ph chromosome/BCR-ABL1 status; most of them by Fluorescent In-Situ Hybridization (FISH) method.

Table 3 Medication use at enrolment
Full size table

Safety profile

Imatinib was generally well tolerated. The most notable adverse effects were some form of myelosuppression: neutropenia and/or thrombocytopenia. Grade 1 or 2 elevation of liver enzymes, edema and weight gain, rashes, nausea, vomiting and diarrhea were other common adverse effects. Patients treated with higher doses of imatinib were more likely to develop grade 1 or 2 nausea, edema, or diarrhea than patients given lower doses of the drug. Neutropenia and/or thrombocytopenia were the most frequent adverse effects and occurred in about 30% of the patients (Table 4).

Table 4 Adverse effects
Full size table

There was a total of 2 deaths and 8 patients lost to follow up. Of the 2 deaths both of them died due to disease progression and subsequent complications. Of the 8 patients lost to follow up, two patients who did not return for follow-up were found to have died due to disease progression and blast crisis. The causes of death of the other 6 patients are not known.

Hematologic and cytogenetic responses

Out of 103 patients, 15% of the patients were already in complete hematologic remission before enrolment in GIPAP. Seventy-eight percent of the patients went into complete hematologic remission within 3 months of starting imatinib and only seven percent of the patients took more than 3 months for complete hematologic remission. Of the 29 patients who had cytogenetic analysis done, 26 (89.66%) patients had major cytogenetic response [20 (68.97%) Complete Response and 6 (20.69%) Partial Response], 2 (6.89%) patients had minor cytogenetic responses, and 1 (3.45%) patient had minimal cytogenetic response (Table 5). The cytogenetic testing was performed between 9 and 61 months (median 26 months) from enrolment in patients who were able to afford testing.

Table 5 Hematologic and cytogenetic analyses
Full size table

Of the 29 patients who underwent cytogenetic analysis, four patients who had had major cytogenetic responses initially showed minimal to minor responses with subsequent FISH analysis. Two of these patients were actually taking reduced doses of imatinib. Another patient temporarily stopped taking imatinib. All three of them responded to dose escalation. The fourth patient responded temporarily to an increased dose but eventually relapsed. This patient who relapsed had been to other hospitals in India and had been treated with interferon and other chemotherapeutic medications before starting imatinib. He had a complete hematologic response only for few months after the beginning of therapy at our hospital. He underwent dose escalation to 600 mg/day initially and then to 800 mg/day, but complete hematologic response was not achieved. Quantitative BCR/ABL by FISH repeated after 6 months showed partial cytogenetic response, but repeat analysis showed lack of response. Subsequent mutation analysis revealed a F359V mutation in the ABL domain of the BCR/ABL oncogene. This patient may have developed secondary resistance due to the mutation. Even with the change of medication to the second generation tyrosine kinase inhibitor nilotinib, his response was not satisfactory. While preparing for this presentation this patient started taking dasatinib and he is responding well to this drug. One more patient who developed secondary hematologic failure showed loss of complete cytogenetic response upon repeat cytogenetic analysis by FISH. Subsequent mutation analysis in this patient revealed L387 M mutation in the ABL domain of BCR/ABL oncogene. This patient is responding to an increased dose of imatinib.

The cumulative survival (including 2 deaths and 8 lost to follow up) is more than 80% (figure 1) with a mean follow up period of 27 months. The overall Survival function (with 4 confirmed CML deaths excluding lost to follow up) is 92% (figure 2) without any difference in mortality between sexes (figure 3). The cumulative survival approaches 100% in patients who underwent cytogenetic analysis (figure 4). Of the 103 CML patients on imatinib, about 90% of the patients did not progress to the accelerated phase or blast crisis with a median follow up of 24 months, and more than 90% of them are still alive.

Figure 1
figure1

Kaplan-Meier of cumulative survival of all patients. This figure shows the cumulative survival of all patients enrolled in GIPAP.

Full size image
Figure 2
figure2

Kaplan-Meier of cumulative survival of patients excluding those lost to follow-up. The cumulative survival of patients, excluding those lost to follow-up, enrolled in GIPAP is shown.

Full size image
Figure 3
figure3

Kaplan-Meier of survival based on gender. The survival of patients, based on gender is shown.

Full size image
Figure 4
figure4

Kaplan-Meier of survival based on cytogenetic or hematologic follow-up. This figure demonstrates that the survival of patients who were able to obtain cytogenetic follow-up was superior to that of patients who only obtained hematologic follow-up.

Full size image

We also looked at differences between characteristics of patients who were analyzed for both cytogenetic and hematologic responses compared to those who were analyzed for only hematologic response. All the patients who died or lost to follow up were from the group who only had hematologic analysis performed, and had not repeated cytogenetic analysis. Patients with cytogenetic analysis had a longer follow up. The cumulative survival approaches 100% in the group who had repeated cytogenetic analysis (figure 4).

Discussion

The prevalence of CML is more common in the 31-50y age group in our study than in most demographic studies. The demographic characteristics between patients with or without cytogenetic analysis were not significantly different. Most of the patients were from Kathmandu and Lalitpur districts. Patients who had cytogenetic analysis repeated had a longer follow up period compared to the patients who had not. The increased number of deaths seen in patients who had not repeated cytogenetic analysis might be related to not being able to assess optimum responses to therapy.

Imatinib is currently the standard frontline therapy for treatment of CML. Druker and colleagues' five year follow-up of chronic phase CML patients treated initially with imatinib showed that the cumulative best rates for complete cytogenetic responses and complete hematologic response were 87% and 98% respectively[11]. An estimated 7% of the patients progressed to accelerated or blast phase. The overall survival rate was 89%, with more patients dying from causes unrelated to CML than their disease. Review of the International Randomized Study of Interferon vs STI571 (IRIS) data suggests that 63% of patients were successfully treated by imatinib at five years, while 37% of patients were no longer receiving the drug. In our study at Patan, 89.7% of our patients had major cytogenetic response (BCR-ABL1/Ph chromosome 0-34%), 6.9% of our patients had minor cytogenetic responses (BCR-ABL1/Ph chromosome 35-64%), and 3.4% of our patients had minimal cytogenetic response (BCR-ABL1/Ph chromosome 65-94%). After a mean follow up of 27 months (median follow up 24 months), CML had not progressed to the accelerated phase or blast crisis in 90% of the patients and more than 90% of the patients are still alive. Our patients' responses to imatinib were similar to those reported in IRIS by Druker and colleagues.

Importantly, our study establishes that imatinib is safe to use in a developing county. Myelosuppression was the most common side effect, and this was easily monitored in our setting with complete blood counts. Our monitoring allowed for appropriate dose adjustments.

Quite often patients on imatinib develop resistance, and the identification of resistance should trigger mutational analysis of BCR-ABL1 kinase domain. Reviews on managing imatinib resistance have been published[18–22]. A few of our patients treated with imatinib relapsed, reflecting the emergence of resistance to imatinib. Although there are multiple mechanisms involved in the emergence of resistance, usually resistance develops because of a mutation in the adenosine triphosphate-binding pocket of the BCR-ABL1 oncoprotein, which makes imatinib binding impossible. Molecular studies of resistant CML cells have been performed and have identified mutations that mediate this effect[23–27]. Several potent inhibitors of imatinib-resistant BCR-ABL1 have been identified and approved for clinical use[28–32]. Two of our patients developed secondary hematologic failure. Subsequent mutation analyses revealed mutations in ABL domain of BCR/ABL oncogene. The patient who had the L387 M mutation responded to dose escalation of Imatinib to 600 mg/day from 400 mg/day[33–36]. Another patient who had F359V mutation did not show response even with dose escalation to 800 mg/day. He was switched to second generation tyrosine kinase inhibitor (TKI) nilotinib but the response was not satisfactory. Failure to achieve major cytogenetic response by 12 months usually defines inadequate response[37]. He was then switched to dasatinib (another second generation TKI) and the response was remarkable. He went into hematological remission within few weeks. Dasatinib seems to be more effective in F359V mutation.

The main problem in our setting is the inability of our patients to obtain regular cytogenetic and molecular analyses as recommended by the standard guidelines in the management of Ph chromosome/BCR-ABL1 positive CML. The high cost of cytogenetic or molecular analysis and lack of availability of diagnostic centers in Nepal where these tests can be easily performed are prohibitive. Quantitative cytogenetic analysis by FISH costs about 7,000 Nepalese rupees (NRs) (US $90) and quantitative molecular analysis of BCR-ABL1/ABL ratio by RT-PCR costs about NRs. 10,000 (US $130). These costs are so high because the test is not done in Nepal; the laboratories only collect the samples and dispatch them to diagnostic facilities in India. Patients are often called back for repeat sample collections because the samples they have collected are lost somewhere in the chain of transport. Only about one-third of the total patients repeated cytogenetic analysis. The cumulative survival is significantly better in this group. Cytogenetic analysis allows detection of inadequate responses or resistance to therapy at an earlier stage of the disease and allows for adjustments in the management including choosing appropriate TKIs or referral for stem cell transplantation. Most of our patients could not afford a second test. So, it is very important that the concerned authorities seriously consider establishing an advanced medical laboratory at an affordable price in our own country.

Despite the fact that our patients hailed from different parts of Nepal, most patients managed to come for follow up regularly with usual hematological and biochemical tests and the compliance with the study medication was good. Interestingly, our patients also had remarkable cytogenetic and hematologic response rates consistent with other well controlled studies done elsewhere. GIPAP has enabled many patients to receive standard of care treatment that would otherwise be prohibitive due to cost in Nepal.

Conclusions

Our study demonstrates that imatinib is safe to use in a developing country. Furthermore, we demonstrate that imatinib is very effective and induced long lasting responses in a high proportion of patients with Ph chromosome/BCR-ABL1 positive CML. Imatinib is well tolerated by our patients. The lack of cytogenetic analysis in the majority of our patients hindered our ability to detect inadequate responses to Imatinib and adjust therapy appropriately.

Abbreviations

CML:

Chronic myeloid leukemia

GIPAP:

Gleevec International Patient Assistance Program

Ph:

Philadelphia

WBC:

White blood cell

FISH:

Fluorescent in situ hybridization

IRIS:

International Randomized Study of Interferon vs STI571

TKI:

Tyrosine kinase inhibitor

NRs:

Nepalese rupees

CFU:

cytogenetic follow-up

HFU:

hematologic follow-up

HR:

hematologic response

CR:

cytogenetic response

CHR:

complete hematologic response

References

  1. 1.

    Rowley JD: Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining. Nature. 1973, 243: 290-293. 10.1038/243290a0.

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Shtivelman E, Lifshitz B, Gale RP, Canaani E: Fused transcript of abl and bcr genes in chronic myelogenous leukaemia. Nature. 1985, 315: 550-554. 10.1038/315550a0.

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Faderl S, Talpaz M, Estrov Z, O'Brien S, Kurzrock R, Kantarjian HM: The biology of chronic myeloid leukemia. N Engl J Med. 1999, 341: 164-172. 10.1056/NEJM199907153410306.

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Kantarjian HM, Talpaz M, Giles F, O'Brien S, Cortes J: New insights into the pathophysiology of chronic myeloid leukemia and imatinib resistance. Ann Intern Med. 2006, 145: 913-923.

    Article  PubMed  Google Scholar 

  5. 5.

    Kurzrock R, Kantarjian HM, Druker BJ, Talpaz M: Philadelphia chromosome-positive leukemias: from basic mechanisms to molecular therapeutics. Ann Intern Med. 2003, 138: 819-830.

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Quintas-Cardama A, Cortes JE: Chronic myeloid leukemia: diagnosis and treatment. Mayo Clin Proc. 2006, 81: 973-988. 10.4065/81.7.973.

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Schiffer CA: BCR-ABL tyrosine kinase inhibitors for chronic myelogenous leukemia. N Engl J Med. 2007, 357: 258-265. 10.1056/NEJMct071828.

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    Cortes J: Natural history and staging of chronic myelogenous leukemia. Hematol Oncol Clin North Am. 2004, 18: 569-584. 10.1016/j.hoc.2004.03.011. viii

    Article  PubMed  Google Scholar 

  9. 9.

    Druker BJ, Sawyers CL, Kantarjian H, Resta DJ, Reese SF, Ford JM, Capdeville R, Talpaz M: Activity of a specific inhibitor of the BCR-ABL tyrosine kinase in the blast crisis of chronic myeloid leukemia and acute lymphoblastic leukemia with the Philadelphia chromosome. N Engl J Med. 2001, 344: 1038-1042. 10.1056/NEJM200104053441402.

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Sawyers CL: Chronic myeloid leukemia. N Engl J Med. 1999, 340: 1330-1340. 10.1056/NEJM199904293401706.

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Druker BJ, Guilhot F, O'Brien SG, Gathmann I, Kantarjian H, Gattermann N, Deininger MW, Silver RT, Goldman JM, Stone RM, Cervantes F, Hochhaus A, Powell BL, Gabrilove JL, Rousselot P, Reiffers J, Cornelissen JJ, Hughes T, Agis H, Fischer T, Verhoef G, Shepherd J, Saglio G, Gratwohl A, Nielsen JL, Radich JP, Simonsson B, Taylor K, Baccarani M, So C, Letvak L, Larson RA: Five-year follow-up of patients receiving imatinib for chronic myeloid leukemia. N Engl J Med. 2006, 355: 2408-2417. 10.1056/NEJMoa062867.

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Druker BJ, Talpaz M, Resta DJ, Peng B, Buchdunger E, Ford JM, Lydon NB, Kantarjian H, Capdeville R, Ohno-Jones S, Sawyers CL: Efficacy and safety of a specific inhibitor of the BCR-ABL tyrosine kinase in chronic myeloid leukemia. N Engl J Med. 2001, 344: 1031-1037. 10.1056/NEJM200104053441401.

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Kantarjian H, Sawyers C, Hochhaus A, Guilhot F, Schiffer C, Gambacorti-Passerini C, Niederwieser D, Resta D, Capdeville R, Zoellner U, Talpaz M, Druker B, Goldman J, O'Brien SG, Russell N, Fischer T, Ottmann O, Cony-Makhoul P, Facon T, Stone R, Miller C, Tallman M, Brown R, Schuster M, Loughran T, Gratwohl A, Mandelli F, Saglio G, Lazzarino M, Russo D, Baccarani M, Morra E: Hematologic and cytogenetic responses to imatinib mesylate in chronic myelogenous leukemia. N Engl J Med. 2002, 346: 645-652. 10.1056/NEJMoa011573.

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    O'Brien SG, Guilhot F, Larson RA, Gathmann I, Baccarani M, Cervantes F, Cornelissen JJ, Fischer T, Hochhaus A, Hughes T, Lechner K, Nielsen JL, Rousselot P, Reiffers J, Saglio G, Shepherd J, Simonsson B, Gratwohl A, Goldman JM, Kantarjian H, Taylor K, Verhoef G, Bolton AE, Capdeville R, Druker BJ: Imatinib compared with interferon and low-dose cytarabine for newly diagnosed chronic-phase chronic myeloid leukemia. N Engl J Med. 2003, 348: 994-1004.

    Article  PubMed  Google Scholar 

  15. 15.

    Sawyers CL, Hochhaus A, Feldman E, Goldman JM, Miller CB, Ottmann OG, Schiffer CA, Talpaz M, Guilhot F, Deininger MW, Fischer T, O'Brien SG, Stone RM, Gambacorti-Passerini CB, Russell NH, Reiffers JJ, Shea TC, Chapuis B, Coutre S, Tura S, Morra E, Larson RA, Saven A, Peschel C, Gratwohl A, Mandelli F, Ben-Am M, Gathmann I, Capdeville R, Paquette RL, Druker BJ: Imatinib induces hematologic and cytogenetic responses in patients with chronic myelogenous leukemia in myeloid blast crisis: results of a phase II study. Blood. 2002, 99: 3530-3539. 10.1182/blood.V99.10.3530.

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Talpaz M, Silver RT, Druker BJ, Goldman JM, Gambacorti-Passerini C, Guilhot F, Schiffer CA, Fischer T, Deininger MW, Lennard AL, Hochhaus A, Ottmann OG, Gratwohl A, Baccarani M, Stone R, Tura S, Mahon FX, Fernandes-Reese S, Gathmann I, Capdeville R, Kantarjian HM, Sawyers CL: Imatinib induces durable hematologic and cytogenetic responses in patients with accelerated phase chronic myeloid leukemia: results of a phase 2 study. Blood. 2002, 99: 1928-1937. 10.1182/blood.V99.6.1928.

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Peggs K, Mackinnon S: Imatinib mesylate--the new gold standard for treatment of chronic myeloid leukemia. N Engl J Med. 2003, 348: 1048-1050. 10.1056/NEJMe030009.

    Article  PubMed  Google Scholar 

  18. 18.

    Borrow J: Guidelines for Mutation Analysis of BCR/ABL Kinase Domain. WMRGL. 2007, 1-32.

    Google Scholar 

  19. 19.

    Goldman JM: How I treat chronic myeloid leukemia in the imatinib era. Blood. 2007, 110: 2828-2837. 10.1182/blood-2007-04-038943.

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Hochhaus A, La Rosee P: Imatinib therapy in chronic myelogenous leukemia: strategies to avoid and overcome resistance. Leukemia. 2004, 18: 1321-1331. 10.1038/sj.leu.2403426.

    CAS  Article  PubMed  Google Scholar 

  21. 21.

    Mauro MJ: Defining and managing imatinib resistance. Hematology Am Soc Hematol Educ Program. 2006, 219-225.

    Google Scholar 

  22. 22.

    Shah NP: Loss of response to imatinib: mechanisms and management. Hematology Am Soc Hematol Educ Program. 2005, 183-187.

    Google Scholar 

  23. 23.

    Branford S, Rudzki Z, Walsh S, Parkinson I, Grigg A, Szer J, Taylor K, Herrmann R, Seymour JF, Arthur C, Joske D, Lynch K, Hughes T: Detection of BCR-ABL mutations in patients with CML treated with imatinib is virtually always accompanied by clinical resistance, and mutations in the ATP phosphate-binding loop (P-loop) are associated with a poor prognosis. Blood. 2003, 102: 276-283. 10.1182/blood-2002-09-2896.

    CAS  Article  PubMed  Google Scholar 

  24. 24.

    Ernst T, Hoffmann J, Erben P, Hanfstein B, Leitner A, Hehlmann R, Hochhaus A, Muller MC: ABL single nucleotide polymorphisms may masquerade as BCR-ABL mutations associated with resistance to tyrosine kinase inhibitors in patients with chronic myeloid leukemia. Haematologica. 2008, 93: 1389-1393. 10.3324/haematol.12964.

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Roche-Lestienne C, Soenen-Cornu V, Grardel-Duflos N, Lai JL, Philippe N, Facon T, Fenaux P, Preudhomme C: Several types of mutations of the Abl gene can be found in chronic myeloid leukemia patients resistant to STI571, and they can pre-exist to the onset of treatment. Blood. 2002, 100: 1014-1018. 10.1182/blood.V100.3.1014.

    CAS  Article  PubMed  Google Scholar 

  26. 26.

    Shah NP, Nicoll JM, Nagar B, Gorre ME, Paquette RL, Kuriyan J, Sawyers CL: Multiple BCR-ABL kinase domain mutations confer polyclonal resistance to the tyrosine kinase inhibitor imatinib (STI571) in chronic phase and blast crisis chronic myeloid leukemia. Cancer Cell. 2002, 2: 117-125. 10.1016/S1535-6108(02)00096-X.

    CAS  Article  PubMed  Google Scholar 

  27. 27.

    Soverini S, Colarossi S, Gnani A, Rosti G, Castagnetti F, Poerio A, Iacobucci I, Amabile M, Abruzzese E, Orlandi E, Radaelli F, Ciccone F, Tiribelli M, di Lorenzo R, Caracciolo C, Izzo B, Pane F, Saglio G, Baccarani M, Martinelli G: Contribution of ABL kinase domain mutations to imatinib resistance in different subsets of Philadelphia-positive patients: by the GIMEMA Working Party on Chronic Myeloid Leukemia. Clin Cancer Res. 2006, 12: 7374-7379. 10.1158/1078-0432.CCR-06-1516.

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Bradeen HA, Eide CA, O'Hare T, Johnson KJ, Willis SG, Lee FY, Druker BJ, Deininger MW: Comparison of imatinib mesylate, dasatinib (BMS-354825), and nilotinib (AMN107) in an N-ethyl-N-nitrosourea (ENU)-based mutagenesis screen: high efficacy of drug combinations. Blood. 2006, 108: 2332-2338. 10.1182/blood-2006-02-004580.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  29. 29.

    Kantarjian H, Giles F, Wunderle L, Bhalla K, O'Brien S, Wassmann B, Tanaka C, Manley P, Rae P, Mietlowski W, Bochinski K, Hochhaus A, Griffin JD, Hoelzer D, Albitar M, Dugan M, Cortes J, Alland L, Ottmann OG: Nilotinib in imatinib-resistant CML and Philadelphia chromosome-positive ALL. N Engl J Med. 2006, 354: 2542-2551. 10.1056/NEJMoa055104.

    Article  PubMed  Google Scholar 

  30. 30.

    Kantarjian HM, Giles F, Gattermann N, Bhalla K, Alimena G, Palandri F, Ossenkoppele GJ, Nicolini FE, O'Brien SG, Litzow M, Bhatia R, Cervantes F, Haque A, Shou Y, Resta DJ, Weitzman A, Hochhaus A, le Coutre P: Nilotinib (formerly AMN107), a highly selective BCR-ABL tyrosine kinase inhibitor, is effective in patients with Philadelphia chromosome-positive chronic myelogenous leukemia in chronic phase following imatinib resistance and intolerance. Blood. 2007, 110: 3540-3546. 10.1182/blood-2007-03-080689.

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    O'Hare T, Eide CA, Tyner JW, Corbin AS, Wong MJ, Buchanan S, Holme K, Jessen KA, Tang C, Lewis HA, Romero RD, Burley SK, Deininger MW: SGX393 inhibits the CML mutant Bcr-AblT315I and preempts in vitro resistance when combined with nilotinib or dasatinib. Proc Natl Acad Sci USA. 2008, 105: 5507-5512.

    Article  PubMed  PubMed Central  Google Scholar 

  32. 32.

    Talpaz M, Shah NP, Kantarjian H, Donato N, Nicoll J, Paquette R, Cortes J, O'Brien S, Nicaise C, Bleickardt E, Blackwood-Chirchir MA, Iyer V, Chen TT, Huang F, Decillis AP, Sawyers CL: Dasatinib in imatinib-resistant Philadelphia chromosome-positive leukemias. N Engl J Med. 2006, 354: 2531-2541. 10.1056/NEJMoa055229.

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Kantarjian HM, Talpaz M, O'Brien S, Giles F, Garcia-Manero G, Faderl S, Thomas D, Shan J, Rios MB, Cortes J: Dose escalation of imatinib mesylate can overcome resistance to standard-dose therapy in patients with chronic myelogenous leukemia. Blood. 2003, 101: 473-475. 10.1182/blood-2002-05-1451.

    CAS  Article  PubMed  Google Scholar 

  34. 34.

    Marin D, Goldman JM, Olavarria E, Apperley JF: Transient benefit only from increasing the imatinib dose in CML patients who do not achieve complete cytogenetic remissions on conventional doses. Blood. 2003, 102: 2702-2703. 10.1182/blood-2003-06-2042. author reply 2703-2704

    CAS  Article  PubMed  Google Scholar 

  35. 35.

    Rea D, Etienne G, Corm S, Cony-Makhoul P, Gardembas M, Legros L, Dubruille V, Hayette S, Mahon FX, Cayuela JM, Nicolini FE: Imatinib dose escalation for chronic phase-chronic myelogenous leukaemia patients in primary suboptimal response to imatinib 400 mg daily standard therapy. Leukemia. 2009, 23: 1193-1196. 10.1038/leu.2009.32.

    CAS  Article  PubMed  Google Scholar 

  36. 36.

    Zonder JA, Pemberton P, Brandt H, Mohamed AN, Schiffer CA: The effect of dose increase of imatinib mesylate in patients with chronic or accelerated phase chronic myelogenous leukemia with inadequate hematologic or cytogenetic response to initial treatment. Clin Cancer Res. 2003, 9: 2092-2097.

    CAS  PubMed  Google Scholar 

  37. 37.

    Tam CS, Kantarjian H, Garcia-Manero G, Borthakur G, O'Brien S, Ravandi F, Shan J, Cortes J: Failure to achieve a major cytogenetic response by 12 months defines inadequate response in patients receiving nilotinib or dasatinib as second or subsequent line therapy for chronic myeloid leukemia. Blood. 2008, 112: 516-518. 10.1182/blood-2008-02-141580.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

Pre-publication history

  1. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2326/10/8/prepub

Download references

Acknowledgements

All authors would like to acknowledge Novartis for providing Imatinib to our patients. ASM would like to recognize the Mayo International Health Program for defraying the costs associated with volunteering in Nepal, and Dr. Mark R. Litzow for his review of this manuscript.

Author information

Affiliations

  1. Department of Internal Medicine, Patan Hospital, Lalitpur, Nepal

    Gyan K Kayastha, Padma Gurung, Paras K Acharya, Buddhi P Paudyal & Arjun Karki

  2. Department of Emergency Medicine, Patan Hospital, Lalitpur, Nepal

    Bruce Hayes

  3. Nick Simon Institute, Lalitpur, Nepal

    Mark Zimmerman

  4. Division of Hematology of the Department of Internal Medicine, and Department of Oncology, Mayo Clinic, Rochester, MN, USA

    Aaron S Mansfield

Authors
  1. Gyan K Kayastha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Padma Gurung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paras K Acharya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Buddhi P Paudyal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bruce Hayes
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mark Zimmerman
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Arjun Karki
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Aaron S Mansfield
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gyan K Kayastha.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

GK was responsible for the acquisition of data. All authors except AK were involved in the care of the patients enrolled in GIPAP. All authors have contributed to the drafting and review of the manuscript. All authors have given final approval for publication of this manuscript.

Authors’ original submitted files for images

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Kayastha, G.K., Gurung, P., Acharya, P.K. et al. Patan hospital experience in treating philadelphia chromosome/BCR-ABL1 positive chronic myeloid leukemia patients with gleevec (imatinib mesylate); the first generation specific tyrosine kinase inhibitor. BMC Hematol 10, 8 (2010). https://doi.org/10.1186/1471-2326-10-8

Download citation

Keywords

  • Imatinib
  • Chronic Myeloid Leukemia
  • Dasatinib
  • Cytogenetic Analysis
  • Nilotinib

BMC Hematology

ISSN: 2052-1839

Contact us