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Fig. 1 | BMC Hematology

Fig. 1

From: A pilot study on the usefulness of peripheral blood flow cytometry for the diagnosis of lower risk myelodysplastic syndromes: the “MDS thermometer”

Fig. 1

Bivariate dot-plots obtained from a normal PB sample, illustrating the analysis procedure used for the immunophenotypic identification, quantification and characterization of neutrophils, monocytes, and CD34+ cells, after excluding cell debris and doublets. Neutrophils (red dots; 61.16%) were selected based on their SSC/FSC characteristics and CD45+lowCD16+high expression. Monocytes (blue dots; 7.64%) were selected based on SSC/FSC and CD45+intCD14+ expression; subsequently, monocytes were separated into classical (light blue dots; 6.88%) and non-classical (either intermediate CD14+highCD16+ or proinflammatory CD14+lowCD16+, dark blue dots; 0.76%) monocyte subsets. Immature CD34+ cells (black dots, 0.02%) were identified based on SSC/FSC and CD45+lowCD34+ expression. Other cells: eosinophils (pink dots; 4.93%); immature granulocytes, including promyelocytes, myelocytes and metamyelocytes (purple dots; 0.27%); lymphocytes and basophils (gray dots; 25.97%). Abbreviations: FSC, forward scatter; PB, peripheral blood; SSC, side scatter

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BMC Hematology

ISSN: 2052-1839

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