Table 1 Primers and conditions for the RT-PCR experiments performed in this study.
Gene | Sequence (5'→3') | T (°C) | Product (bp) |
|---|---|---|---|
IL-2 | GCAACTCCTGTCTTGCATTG AATGTGAGCATCCTGGTGAG | 59 | 173 |
IFN-γ | AGCTCTGCATCGTTTTGGGTTC CAAATATTGCAGGCAGGACAACC | 64 | 492 |
IL-4 | CTGTGCTCCGGCAGTTCTAC ACGTACTCTGGTTGGCTTCC | 58 | 176 |
IL-5 | GCTTCTGCATTTGAGTTTGCTAGCT TGGCCGTCAATGTATTTCTTTATTAAG | 59 | 291 |
IL-10 | ACCCAGTCTGAGAACAGCTGC GTTCACATGCGCCTTGATGTCT | 61 | 260 |
β2m | CCCCCACTGAAAAAGATGAG TCACTCAATCCAAATGCGGC | 56 | 150 |