Figure 3
Protein expression in FA and control cells treated with cisplatin. 10 μg protein from indicated cell extracts were immunoblotted with specific antibodies directed against indicated proteins; A) BAF170, CDC25a, PMS2, RGS2 and β-tubulin; B) p21waf1/Cip1. Extracts were from HSC72 and HSC536 cells transfected with empty vector or with vector expressing correcting cDNA as indicated. Cells were one hour pulse treated with discriminating concentrations of cisplatin; HSC72 with 5 μM and HSC536 with 1 μM. β-tubulin was used as additional loading control.