| LR-MDS (n = 14) | Controls (n = 14) |
---|
Neutrophil immunophenotypic score 1 (0–8) |
Score | 5 (0–8) | 0 (0–1) |
Score rank (Σ) | 59 | 1 |
Score classes |
0 (no dysplasia) | 1 (7%) | 13 (93%) |
1 (possible dysplasia) | 0 (0%) | 1 (7%) |
2–3 (mild dysplasia) | 3 (21%) | 0 (0%) |
4–6 (moderate dysplasia) | 9 (64%) | 0 (0%) |
7–8 (severe dysplasia) | 1 (7%) | 0 (0%) |
Monocyte immunophenotypic score 1 (0–2) |
Score | 1 (0–2) | 0 (0–1) |
Score rank (Σ) | 15 | 2 |
Score classes |
0 (no dysplasia) | 2 (14%) | 12 (86%) |
1 (possible dysplasia) | 8 (57%) | 2 (14%) |
2 (dysplasia) | 4 (29%) | 0 (0%) |
Myeloid immunophenotypic score 1 (0–10) |
Score | 6 (0–8) | 0 (0–1) |
Score rank (Σ) | 74 | 3 |
Score classes |
0 (no dysplasia) | 1 (7%) | 11 (79%) |
1 (possible dysplasia) | 0 (0%) | 3 (21%) |
2–4 (mild dysplasia) | 3 (21%) | 0 (0%) |
5–7 (moderate dysplasia) | 7 (50%) | 0 (0%) |
8–10 (severe dysplasia) | 3 (21%) | 0 (0%) |
- Abbreviations: LR-MDS lower risk myelodysplastic syndromes, PB peripheral blood
- Results are expressed as absolute and (relative) frequencies or as median (range) values
- Percentages were approximated to the closest full unit
- Neutrophil score: The following parameters were considered: FSC, SSC, CD10, CD11b, CD11c, CD13, CD16, and CD45. Each abnormally low parameter (< mean – 2D of the values observed in controls) was scored with 1 point. Values within the mean ± SD or above the mean + 2SD were scored with “0”
- Monocyte score: The following parameters were considered: % of CD16+ monocytes and % of CD56+ monocytes. These parameters were scored as follows: CD14 + CD56+ monocytes: ≤18% (0 points); > 18% (1 point); CD14 + CD16+ monocytes: ≥5% (0 points); < 5% (1 point)
- Myeloid score: obtained by adding up the neutrophil and the monocytic score achieved for each patient
- The following score rankings were obtained for each of the parameters analyzed: Patients: FSC = 10; CD11b = 10; CD11c = 10; CD10 = 9; SSC = 8; CD13 = 4; CD15 = 0; CD16 = 6; CD45 = 2. Controls: CD15 = 1; CD45 = 1; other parameters = 0