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Table 4 HMEC-1 cells secrete urokinase-type plasminogen activator and plasminogen activator inhibitor type 1

From: Endothelial fibrinolytic response onto an evolving matrix of fibrin

μg/mL per cell

Model 1

Model 2

uPA×10−9 (−RGD)

12.7*,** (11.2–14.1)

6.2* (5.0–7.4)

uPA×10−9 (+RGD)

7.5** (5.9–9.1)

17.3 (15.0–19.6)

PAI-1×10−6 (−RGD)

7.87** (6.65–9.10)

5.97 (4.50–7.44)

PAI-1×10−6 (+RGD)

7.38** (6.54–8.23)

5.08 (3.01–7.16)

  1. Urokinase and PAI-1 were quantified by ELISA in HMEC conditioned-medium obtained in the presence of fibrin. Fibrin was formed on the top of the cells monolayer (model 1) or cells were seeded on the top of fibrin film (model 2). The basal secretion of uPA was 7.0 ± 0.5 × 10−9 μg/mL per cell, and that of PAI-1 6.7 ± 0.5 × 10−6 μg/mL per cell. The quantity of uPA and PAI-1 secreted was normalized to the number of cells. Results are expressed as the mean and the 95 % confidence interval (CI) in brackets
  2. *p < 0.05 uPA secretion without RGD (−RGD) compared to that with RGD (+RGD) for model 1
  3. **p < 0.05 uPA secretion without RGD of model 1 compared to that of model 2, and with RGD in model 1 compared to that in model 2