The expression of embryonic globin mRNA in a severely anemic mouse model induced by treatment with nitrogen-containing bisphosphonate

Table 1 Used primer sequence

Name	Sequence	Annealing(°C) Product size
Actin	F: 5’-GCGTGACATTAAAGAGAAGCTG-3’	60
Actin	R: 5’-CTCAGGAGGAGCAATGATCTTG-3	376 bp
Hba	F: 5’-CTCTCTGGGGAAGACAAAAGCAAC-3’	55
Hba	R: 5’-GGTGGCTAGCCAAGGTCACCAGCA-3’	334 bp
Hbb1	F: 5’-CACAAACCCCAGAAACAGACA-3’	55
Hbb1	R: 5’-CTGACAGATGCTCTCTTGGG-3’	529 bp
Hba-x	F: 5’- CTGTCTGCTGGTCACAATGG -3’	55
Hba-x	R: 5’- GGGAGGAGAGGGATCATAGC -3’	165 bp
Hbb-bh1	F: 5’-TGGACAACCTCAAGGAGACC-3’	55
Hbb-bh1	R: 5’- TGCCAGTGTACTGGAATGGA -3’	231 bp
Hbb-y	F: 5’-CTTGGGTAATGTGCTGGTGA-3’	55
Hbb-y	R: 5’-GTGCAGAAAGGAGGCATAGC-3	183 bp
Klf1	F: 5’-CCTCCATCAGTACACTCACC-3’	55
Klf1	R: 5’-CCTCCGATT TCAGACTCACG-3’	150 bp
Klf2	F: 5’-CCAAGAGCTCGCACCTAAAG-3’	55
Klf2	R: 5’-GTGGCACTGAAAGGGTCTGT-3’	155 bp
Bcl11a	F: 5’-AACCCCAGCACTTAAGCAAA-3’	55
Bcl11a	R: 5’-ACAGGTGAGAAGGTCGTGGT-3’	122 bp
Gata1	F: 5’- ACCACTACAACACTCTGGCG -3’	60
Gata1	R: 5’- CAAGAACTGAGTGGGGCGAT -3’	452 bp
bh1-globin promoter	F: 5’-GGACAGGTCTTCAGCCTCTTGA-3’	56
bh1-globin promoter	R: 5’-CAGATGCTTGTGATAGCTGCCT-3’	123 bp
Ey-globin promoter	F: 5’-TGCTTCTGACACTCCTGTGATCA-3’	56
Ey-globin promoter	R: 5’-GGGTTTTTTCCTCAGCAGTAAAGT-3’	79 bp

ISSN: 2052-1839