Fig. 3

Erythropoiesis in the BM and liver. a Flow cytometric analysis of erythroid cells in the BM at 5 days after the treatment. In the controls animals, TER119 single-positive and TER119 and CD71 double-positive erythroid lineage cells comprised the majority of the cells. A significant decrease in the number of TER119-positive cells was induced by the NBP injection. In the animals treated with both NBP and PHZ (NBP + PHZ), the number of TER119 and CD71 double-positive (erythroid lineage) cells was markedly increased despite treated with NBP. b Flow cytometric analysis of erythroid cells in the liver at 5 days after the treatment. The number of TER119 single-positive cells was decreased in animals treated with both NBP and PHZ. TER119 and CD71 double-positive cells were rare in the control animals but were increased by treatment with NBP. The number of TER119/CD71-double-positive erythroblasts was decreased with the NBP treatment. The number of TER119/CD71 double-positive erythroblasts was higher in the group treated with both NBP and PHZ. c Immunohistochemical detection of TER119-positive cells in the BM at 5 days after the treatment. TER119-positive cells were significantly decreased by NBP treatment alone, but a decline in erythropoiesis was not detected in the group treated with both NBP and PHZ (NBP + PHZ). d Histological analysis of the livers of mice treated with NBP and PHZ at 5 days after NBP injection. The left panel shows HE staining. Mononuclear cells accumulated in the liver and formed numerous clusters (arrow). The middle and right panel show immunohistochemistry for TER119 and CD71, respectively. The cells that formed clusters in the liver were TER119- and CD71-positive erythroblasts (arrow). Bars indicated 50 μm (c) and 25 μm (d). The error bars indicate the SEM (a and b)