Fig. 2

Morphological analysis of peripheral blood. a Blood smears stained with May-Grünwald Giemsa stain in control animals and the animals treated with NBP-alone (at 5 days after the NBP) and both NBP and PHZ (at 3 days after PHZ). Nucleated erythroid cells were easily detected in the NBP + PHZ mice (arrows). b Flow cytometry analysis of erythroid lineage cells in the peripheral blood at 5 days after NBP injection. The majority of cells in the control and NBP-alone groups were single-positive TER119 cells at 5 days after NBP injection, whereas the number of TER119 and CD71 double-positive cells was significantly increased by treatment with NBP and PHZ (NBP + PHZ) at 3 days after PHZ injection. c Immunohistochemical detection of CD71-positive cells in peripheral blood of splenectomized mice treated with NBP and PHZ (3 days after PHZ). CD71-positive reticulocytes were observed (arrows in left panel), and CD71-positive nucleated cells also existed in the blood vessel (arrows in right panel). Asterisks indicate statistical significance, and NS denote no significance versus the control. Bars indicate 10 μm (a) and 25 μm (c). The error bars indicate the SEM