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Fig. 8 | BMC Hematology

Fig. 8

From: A quantitative assessment of the content of hematopoietic stem cells in mouse and human endosteal-bone marrow: a simple and rapid method for the isolation of mouse central bone marrow

Fig. 8

Engraftment of the cBM and eBM by human cells in immunodeficient mice. uPA-NOG mice analyzed 308 days after transplant showed engraftment by CD34+/++ (large rectangular region) and CD133+CD34++ (smaller gated subset) cells in both the cBM and eBM (a). The frequencies of these precursor populations found among all live, single cells and human CD45+ cells are shown using box plots (n = 5) (b). Engraftment of various HSC compartments in irradiated NSG mice is indicated (c). Rectangular regions define CD34++CD133+ and CD34++CD133− precursors in the cBM (top row) and eBM (bottom row). The ratio of gated CD133+ to CD133− cells are indicated for cBM and eBM. The expression HLA-DR, CD13 and CD33 on CD34++CD133+ and CD34++CD133− precursors from cBM and eBM is shown in histogram plots (d). Staining with the indicated antibody is shown with blue shading, whereas staining with an isotype-matched control antibody is shown in black outline

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BMC Hematology

ISSN: 2052-1839

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