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Fig. 7 | BMC Hematology

Fig. 7

From: A quantitative assessment of the content of hematopoietic stem cells in mouse and human endosteal-bone marrow: a simple and rapid method for the isolation of mouse central bone marrow

Fig. 7

Loss of some cell-surface markers on hematopoietic precursors owing to enzymatic digestion. Flow cytometric analysis was performed on cBM before and after enzymatic digestion was performed as for the isolation of eBM. The data shown in the dot plots are gated on live, single, low side-light scatter cells (not shown) as well as Lin− cells gated as indicated. Expression of CD133 is indicated using blue dots in all dot plots. Numbers represent the frequencies of Lin− cells represented by the gated populations. Overlay histogram plots show the effects of enzyme digestion on antigen expression. The expression of Lin antigens and CD34 are shown on live, single cells (gating not shown), whereas expression of the remaining antigens are shown on live, single cells expressing CD34 as indicated. BM cells were obtained from a 20 weeks’ gestation fetus

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