Figure 2
From: Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR
Evaluation of efficiency of each target CNV assay and reference assay RNaseP. Quantification cycle is plotted as a function of DNA concentration. Standard curves were generated using a 10-fold serial dilution of DNA. The resulting equations were: y = 3,2868x + 36,147, y = 3.1712x + 35.587, y = 3.3393x + 36.224, y = 3.2791x + 35.417 and y = 3.4487x + 36.523 for the HBA1, HBA2, HBA3.7, HS-40 and Rnase P assays, respectively. All curves showed a highly linear correlation (r2) of 1.0, 0.999, 0.999, 0.999 and 1.0, respectively.