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Table 1 Primer sequences for PCR amplification of ELA2exon 2–5

From: Can mutations in ELA2, neutrophil elastase expression or differential cell toxicity explain sulphasalazine-induced agranulocytosis?

ELA2 target sequence

Primers

Exon 2 F

5'-tgtaaaacgacggccagtgggaggggacaggctccttgg-3'

Exon 2 R

5'-caggaaacagctatgaccaccgggacgcggggtccgagc-3'

Exon 3 F

5'-tgtaaaacgacggccagtcaggcccgtcgccggatggg-3'

Exon 3 R

5'-caggaaacagctatgacctccgtcgcagcctccaccct-3'

Exon 4 F

5'-tgtaaaacgacggccagtgtgacgcgctgacgatctgt-3'

Exon 4 R

5'-caggaaacagctatgaccgcagtaccgggctgggagcg-3'

Exon 5 F

5'-tgtaaaacgacggccagtcagtccagcttccccacctt-3',

Exon 5 R

5'-caggaaacagctatgaccgacctactgaccattttcaac-3'

  1. PCR primers sequences for ELA2 exon 2–5, for following sequencing reactions with BigDye primer, Applied Biosystems.
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