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Figure 2 | BMC Hematology

Figure 2

From: Can mutations in ELA2, neutrophil elastase expression or differential cell toxicity explain sulphasalazine-induced agranulocytosis?

Figure 2

Western blot analysis of human neutrophil elastase (hNE) expression after sulphasalazine exposure U937 cells were incubated for 24 h with 0, 125 and 250 μM sulphasalazine, followed by cell lysis and protein isolation. 20 μg of protein was applied in each lane, transferred to nitrocellulose membrane and incubated with human neutrophil elastase antibody.

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