Table 1 Oligonucleotides primers used for PCR analysis
From: Fanconi anemia genes are highly expressed in primitive CD34+hematopoietic cells
| Genes | Primer sequences | Position on cDNA | Size of PCR fragment | Accession number | |
|---|---|---|---|---|---|
| Fanca | S | 5'-tccttgtcagcgagatcatg | 2955 | 457 | XM125090 |
| A | 5'-cctgaagaagtggatggtaat | 3412 | |||
| Fancc | S | 5'-cttacggtgctccatgtcttg | 1337 | 653 | NM007985 |
| A | 5'-ctgagcagcatcaggagacgg | 1990 | |||
| Fancd1/Brca2 | S | 5'-agccccatgcagcctccacttg | 9394 | 259 | NM009765 |
| A | 5'-cggggacggtaagggcagcc | 9653 | |||
| Fancd2 | S | 5'-gccgggctttgagatgatc | 913 | 418 | AK019136 |
| A | 5'-ccgtgcagggaccagaaca | 1331 | |||
| Fance | S | 5'-ggccctcgtctccttctgtg | 1103 | 246 | AK013325 |
| A | 5'-gctctgctgctctgtgatctg | 1349 | |||
| Fancf | S | 5'-cacgaggtccctacacagatggaggacatg | ND | 228 | *H.Joenje |
| A | 5'-agcctgggaactgagaatctactctagcac | ||||
| Fancg | S | 5'-tccccacccacctcctctctag | 1176 | 317 | AY049715 |
| A | 5'-caggcagggtccgaaagagcag | 1493 | |||
| Brca1 | S | 5'-cagcgggcgaccatgaagtataa | 4260 | 304 | XM126697 |
| A | 5'-ggccttcccatccctgactcgt | 4564 | |||
| Hprt | S | 5'-agtcccagcgtcgtgattag | 100 | 269 | NM013556 |
| A | 5'-aggaatggatctatcactat | 369 | |||
| CD34 | S | 5'-atgcaggtccacagggacacg | 51 | 220 | BC006607 |
| A | 5'-ctgtcctgatagatcaagtag | 271 | |||