Figure 4
From: Fanconi anemia genes are highly expressed in primitive CD34+hematopoietic cells
Comparative analysis of Brca1 and Brca2/Fancd1 gene expression in hematopoietic cells from Fancc -/- mice. (A) Representative gel electrophoresis of 10 ul and 5 ul of RT-PCR products using specific Brca1 or Brca2/Fancd1 and Hprt primers, respectively, from Fancc -/- BM cells, BM; lineage and Thy1.2-depleated, Lin-; sorted LTS-CD34+, CD34+; and LTS-CD34-, CD34- cells. (B) Quantitative comparison of the expression levels of Brca1 and Brca2/Fancd1 genes in hematopoietic cell populations from Fancc+/+ and Fancc-/- mice. Bars represent the mean value from 4 to 10 separate determinations. Absence of SEM bars represents values to low to appear. Significant differences between Fancc -/- and Fancc +/+ cells where *p < 0.05 and ** p < 0.005.