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Figure 3 | BMC Hematology

Figure 3

From: Fanconi anemia genes are highly expressed in primitive CD34+hematopoietic cells

Figure 3

Comparative analysis of Fanc gene expression in hematopoietic cells from Fancc -/- mice. (A) Representative gel electrophoresis of 10 ul (* represents 5 ul) of RT-PCR products from Fancc -/- BM cells, BM; lineage and Thy1.2-depleated, Lin-; sorted LTS-CD34+, CD34+; and LTS-CD34-, CD34- cells using specific Fanc and Hprt primers. (B) Quantitative comparison of the expression levels of Fanc genes in hematopoietic cell populations from Fancc+/+ and Fancc-/- mice. Bars represent the mean value from 2 to 10 separate determinations with the exception of Fance and Fancf in CD34- cells where values represent one determination. Fancg expression in CD34- cells was undetectable in both Fancc+/+ (n= 4) and Fancc-/- cells (n= 4). Fancc +/+ expression ratios are identical to those in Figure 2. Significant differences between Fancc -/- and Fancc +/+ cells where * p < 0.05, ** p < 0.005 and † p < 0.001.

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