Figure 1

Sorting regions used for LTS-CD34 ⁺ and LTS-CD34 ^- cell purification. (A) Lineage and Thy1.2 positive cells were removed from murine BM cell preparation as described in materials and methods. Lin^-Thy1.2^- cells were sorted on the basis of CD34 and Sca-1 expression using R1 and R2 sorting windows. (B) Reanalysis of sorted Lin^-Thy1.2^-Sca-1⁺CD34^- and Lin^-Thy1.2^-Sca1⁺CD34⁺ cells. (C) Representative gel electrophoresis of RT-PCR products in sorted Lin^-Thy1.2^-Sca1⁺CD34^- and Lin^-Thy1.2^-Sca1⁺CD34⁺ cells from wild-type mice using either murine CD34 or Hprt primers.