Table 1 ZFN targeting and cleaving within the hemoglobin, beta ( β A )-gene
Zinc Finger Nuclease (ZFN) | Left Fn; triplet- α-Helix | Right Fn; triplet- α-Helix |
|---|---|---|
-targeting HBB ( β A)-DNA at 5′ Sites | ||
ZFN-unknown-SP-5-1 | Â | Â |
5′_82 cCGTTACTGCCCTGT GGGGCAAGGt 106_′3 | F1; RNITLVR; (ACG) | F1; RNEHLKV; (AGG) |
5′_82 gGCAATGACGGGACACCCCGTTCCa 106_′3 | F2; QRSSLVR; (GTA) | F2; QSTTLKR; (GCA) |
| Â | F3; QDNTLRR; (GCA) | F3; RTEHLAR; (GGG) |
-targeting HBB ( β A )-DNA at 3′ Sites | ||
ZFN-unknown-SP-7-1 | Â | Â |
5′_1333 cTTCCTCCCACAGCTCCTGGGCAACGt 1359_′3 | F1; QASNLLR; (GAA) | F1; RSQTLAQ; (ACG) |
5′_1333 gAAGGAGGGTGTCGAGGACCCGTTGCa 1359_′3 | F2; RQDNLGR; (GAG) | F2; QSTTLKR; (GCA) |
| Â | F3; RMDHLAG; (TGG) | F3; RSDHLSL; (TGG) |
ZFN-unknown-SP-6-1 | Â | Â |
5′_1334 tTCCTCCCACAGCTCCTGGGCAACGt 1359_′3 | F1; RTDRLIR; (GGA) | F1; RSQTLAQ; (ACG) |
5′_1334 aAGGAGGGTGTCGAGGACCCGTTGCa 1359_′3 | F2; QSAHLKR; (GGA) | F2; QSTTLKR; (GCA) |
| Â | F3; RNTALQH; (GTG) | F3; RSDHLSL; (TGG) |