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Table 1 The primer sequences, exons amplified and the restriction enzymes used in the PCR/RFLP procedures to detect five G6PD deficient mutations and the silent one [2023]

From: Prevalence and molecular characterization of Glucose-6-Phosphate dehydrogenase deficient variants among the Kurdish population of Northern Iraq

Variant Primers Exon amplified Restriction Enzyme
Mediterranean
(563 C→T)
5'GGT GAG GCT CCT GAG TAC CA 3' VI MboII
  5'AGC TGT GAT CCT CAC TCC CC 3'   
Chatham
(1003 G→A)
5'CAA GGA GCC CAT TCT CTC CCT T 3' IX BstXI
  5'TTC TCC ACA TAG AGG AGG ACG GCT GCC AAA GT3'   
A-
(202 G→A)
5'CGT GTC CCC AGC CAC TTC TA 3' III-V NlaIII
  5' CAC GCT CAT AGA GTG GTG GG 3'   
Cosenza
(1376 G→C)
5'GCA GCC AGT GGG ATC AGC AAG 3'
5' GGC AAG GAG GGT GGC CGT GG 3'
XI-XIII Bsu361
Aures
(143 T→C)
5' CAG CCA CTT CTA ACC ACA CAC Ct 3'
5' CCG AAG TTG GCC ATG CTG GG 3'
III Mbo I
Silent
(1311 C→T)
5' TGT TCT TTC AAC CCC GAG GAG T 3'
5' AAG ACG TCC AGG ATG AGG TGA TC 3'
Part X-XI Bcl I